Four time league champions became the first big casualty of the Uganda Cup round of 64 after being ejected by lower division side KJT.
Nicholas Kabonge scored the only goal of the game in 24th minute at Mandela National stadium, Namboole to earn Paul Nkata his first official defeat since his return to the helm at the club.
Maroons, another top tier side also crashed out thanks to a 1-0 defeat against Water.
Elsewhere, Bright Stars, Bul and Soana all made it to the round of 32 where they join Express.
Fred Kajoba’s side edged Vura Stars 1-0 in Vura, Soana pummeled Ntoda 3-0 in Ntungamo while Kefa Kisala’s Bul were 1-0 winners over Sun City.
League strugglers UPDF also matched to the next round with a slim 1-0 win over Busia Fisheries while Onduparaka and Masavu progressed without breaking calories after their opponents failed to show up.
On Saturday, Express eliminated Kachumbala 6-0 in a one side game at Mutesa II stadium, Wankulukuku.
Action in the same round continues over the next weekend with Vipers, Police and SC Villa all involved.
Results for Round of 64 Games played on Saturday and Sunday
Express 6-0 Kachumbala Rock Stars
Kansai Plascon 6-1 Butyaba Red Stars
Pallisa Young 0-5 Nyamityobora
Ntoda 0-3 Soana
City Lads 0-3 Kira United
Amuka Bright Light 3-1 Saviours FC
Rushere 2-0 Kamuli
Bugwere 0-2 Ndejje University
Ntinda United 2-0 Young Elephants
Kabale Sharp 1-2 Busula
URA 0-1 KJT
Water 1-0 Maroons
Vura Stars 0-1 Bright Stars
Sun City 0-1 BUL
Busia fisheries 0-1 UPDF
Kireka United 1 (3)- 1 (4) Arua Tigers
Kyetume 0 (2) – 0 (4) Luwero United
Nabitende United 0 (4)- 0(3) Bumate United
Kitara FC 1(7)-1(6)Koboko Rising stars
Masavu Vs JMC Hippos (Away team failed to show up)
Lira United Vs Kataka (Away team failed to show up)
Onduparaka Vs Light S.S (Away team failed to show up)
Synergy Vs Bukedea (Away team failed to show up)